Composite

Part:BBa_K419012

Designed by: YANG TUNG CHIN   Group: iGEM10_TzuChiU_Formosa   (2010-10-24)

CrtEBIY ligate on pSB1C3

The registry part BBa_K419012 is created by merging iGEM 2009 Cambridge biobrick BBa_k274200 with iGEM 2010 standard backbone pSB1C3. This biobrick belongs to the synthesis system of 2010 TzuChi_U Formosa team’s project – Nutrient synthesizer, in order to produce beta-carotene.

The genes in BBa_K419012 is crtE,B,I,Y respectively. The constitutive promoter in BBa_K419012 will drive the downstream gene crtE,B,I,Y respectively in order to convert geranylgeranyl pyroiphosphate (GPP) into beta-carotene.

Photo1.jpg

iGEM Cambridge team characterize their part by defining the best absorbance ratio of a single colony. While we define our part BBa_K419012’s by measuring the beta-carotene produced by a single DH5α over a period of time. The productivity of beta-carotene per cell over six hours period is 1.425X10^-9 microgram.

Please refer to the Experience page for more information.

Reference: Farmer WR and Liao JC. Improving lycopene production in Escherichia coli by engineering metabolic control. Nature Biotechnology 18, 533-537 (2000)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1573
    Illegal NgoMIV site found at 1703
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


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